titration of rinderpest virus and its neutralizing antibody

Titration of Rinderpest Virus and Its Neutralizing Antibody

Use of Vero cells for titration of rinderpest virus and its neutralizing antibody.

Primary bovine embryonic kidney cell was successfully replaced by the green monkey cell line (Vero) for the isolation and seroneutralization of rinderpest virus.

Estimation of virus - neutralizing antibody in

Virus-neutralizing antibodies are seldom estimated in routine diagnostic laboratories because methods involving replicate series of tubed cell monolayers are so demanding of time and materials. Simplified methods for performing neutralization tests have been described, but these were generally developed for specific purposes and have required various forms of specialized apparatus. One of the e...

Estimation of virus-neutralizing antibody in microplates.

A simple and rapid method for the titration of virus-neutralizing antibody is described. The micro-apparatus and reagents are readily available from commercial sources. Results are included from selected cases which illustrate various conditions of immune status and response.

Establishment of titration system for human herpesvirus 6 and evaluation of neutralizing antibody response to the virus.

The susceptibilities of seven T-cell lines to human herpesvirus 6 (HHV-6) infection were examined. MT-4 cells were the most susceptible of these lines to infection with this virus. Therefore, chemically adhered MT-4 cell monolayers were used for infectious HHV-6 assay by indirect immunofluorescent-antibody (IFA) staining. When cell monolayers were fixed 30 to 45 h postinfection, the foci staine...

Neutralizing antibody responses to varicella-zoster virus.

Neutralization of varicella-zoster (V-Z) virus by human sera and immune rhesus monkey sera was enhanced by fresh guinea pig complement. There was no marked difference in the degree to which complement enhanced neutralization by sera from current V-Z virus infections and sera from long-past varicella infections. Immunoglobulin G neutralizing antibody in sera from varicella cases was enhanced by ...